Prabhleen playfully refers to the process of maintaining mammalian cell cultures, commonly used for research by biologists to study various biological processes. To set up a cell culture, cells need to be isolated from the organ or tissue of interest either mechanically or with the use of enzymes. They are then cultured or grown in a flask which has a growth medium that contains adequate nutrients for the cell to grow. The resulting batch of cells are called primary cell cultures. However, because the cells divide, they need to be transferred into a new vessel with fresh growth medium where they will divide further. At this stage, it is called a cell line. Cell lines can either be finite or immortal. Finite cell lines typically perish after a few subcultures while immortal cell lines, as their names suggest, are immortal. One commonly used immortal cell line is the HeLa Cell line, which is the oldest such cell line, the cells of which were obtained on February 8, 1951, from Henrietta Lacks, a 31 year old African American Woman, without her knowledge, which was a common practice at the time. The HeLa cell line has contributed immensely to the study of cervical carcinoma.