
Western Blot: A Poem
PALLAVI
Western blot, Western blot
Western blot, Western blot
इतना क्यों सताते हों?
Why do you cause so much trouble?
Result लाने में एक नहीं, दो तो कभी तीन दिन लगाते हो।
To obtain results, you take a day or two and sometimes even three!
क्या आपको खबर है कि आपको चलाने वाले ढेर सारे
Do you know, to make you work, the ones who work with you,
Buffers बनाते-बनाते होती हमारी श्याम हैं!
Make a ton of buffers, so much so that the night arrives in all the making!
कभी distilled water, reagents तो कभी pH पर उठते कई सवाल हैं।
Sometimes distilled water or reagents, other times pH is questioned.
कभी addition मे हो जाए अगर हमसे कोई भूल,
If ever we make a mistake in the addition,
कर देते हो हमारे सारे bands आप झट से गुल !
You make all our bands disappear with your lies!
बिजली में fluctuations आपको जरा भी ना भाए,
You do not like it when there is even the slightest fluctuation in electricity,
कर देते हो सारे bands दांये से बांये।
You mix up the position of all the bands.
Rocker की घंटी सुन, हम उठ खडे हो जाए,
Upon listening to the bell of the rocker, we stand straight immediately,
हर दस मिनट बाद आपको नहलाए- फुसलाए |
Every ten minutes we have to wash you and coax you.
Chemidock में शक्ल दिखाने के लिए लेते हो आप घंटे
To show yourself in the chemidock, you take hours
Booking के मारे हो जाए हम labmates में झगड़े और तंटे।
Because of the booking, we labmates end up fighting and quarrelling
Protein तो ला देते हो ठीक-ठाक कभी कबार
Sometimes you do bring out the proteins to shine on alright
लेकिन equal actin पें उठते कई सवाल हैं।
But, whether many questions are raised on the equal actin.
आपको मालूम नहीं की antibody को पाने के लिए करते हम कई जुगाड हैं।
Do you know how much jugaad we put in to obtain each antibody!
कभी आ जाते हो ढंग से तो बरसातें हो खुशियाँ
When you manage to come up alright, you bring abundant happiness
हजार और हो जाओ पेपर में publish तो करतें हो खुशियों की बौछार !!
And if you manage to become publication-worthy, our joy knows no bounds.
Translated by Ipsa Jain and Ipshita Raj

About Pallavi
Pallavi Shewale is a PhD Scholar at the department of Biotechnology, Savitribai Phule Pune University.

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